potato_soil_treatment_microbiome

Organic amendments to potato soils inconsistently enrich yield-associated soil microbiota across growing regions of the continental US

Authors: Scott A. Klasek, James E. Crants, Brenda K. Schroeder, Kenneth E. Frost, Carl J. Rosen, Linda L. Kinkel

This work is part of the Potato Soil Health Project, administered by the University of Minnesota and funded by the USDA National Institute of Food and Agriculture Specialty Crops Research Initiative (SCRI).

The manuscript associated with this archived repository is currently under review.
For additional context on soil microbiomes across this study system, please see our Phytobiomes manuscript from 2024 and its associated page.

Sequence Data Availability:
Raw 16S and ITS amplicon sequence reads associated with this study are publicly available as NCBI BioProject PRJNA992073.

Reproducible Workflow:
Data analyses associated with this study are available at this repository. For amplicon processing scripts, please see the page for our Phytobiomes manuscript. Sequence data were processed in the same way across both studies.

By topic:

merging phyloseqs, library depths, basic ordinations
variance partitioning analysis
variance partitioning visuals- including figures 1 and 4
robust linear regressions of ASVs to tuber yields
variance partitioning visuals part II: including figures 2, 3, and supplemental figures S1 and S2
microbiome modeling: SEMs and LMs
microbiome modeling results
scenario summary and ASV barplots, including figures 5 and 6
additional metadata and PLFA, including supplemental figure S3

Licensing info, if you want to use the code here.